Reagents

RSRF encourages researchers to share reagents.  If you have an animal model, expression plasmid, antibodies, permanent cell lines, assay or other reagent you would like to make available to the Rett scientific community please contact Monica Coenraads, RSRF Director of Research at monica@rsrf.org.

Antibodies are available through Upstate Biotechnology in Lake Placid, NY and Santa Cruz Biotechnology.

Weidong Wang of the National Institute on Aging, NIH has developed a polyclonal MeCP2 antibody generated in rabbit. The antibody is suitable for immunoprecipitation and immunoblotting of MeCP2 from human, mouse and rat. For more information please contact Dr. Wang at WangW@grc.nia.nih.gov. Click here to read the protocol for this antibody.

Janine LaSalle of UC Davis has developed a C-terminal chicken anti-MeCP2 antibody. For more information please contact Dr. LaSalle at jmlasalle@ucdavis.edu. Click here to read the protocol for this antibody.

Christopher Woodcock of UMass is making available mutant human MeCP2 plasmids. All have been sequenced and produce good yields of protein. Please contact Dr. Woodcock for the protocols. The following plasmids are available:

• Wildtype
• E397K
• F155S
• R106W
• R294X
• H370X
• R453X
• R106W + R294X
• R106W + H370X

The lab of Charlotte Kilstrup-Nielsen and Nicoletta Landsberger of the Universita dell'Insubria (Italy) has different expression vectors for prokaryotic and mammalian cells of wild type and mutated MeCP2 from human, mouse and Xenopus. A more detailed description will follow.  In the interim, for more information please contact Dr. Landsberger at landsben@uninsubria.it